Protective effect of melatonin on γ radiation-induced sperm malformations in C57BL/6 male mice
Warsaw Medical Physics Meeting 2015
Ritu Kushwaha, Dhruv K Nishad, Aseem Bhatnagar
Background: Melatonin, a compound known for its antioxidant properties, scavenges free radicals and counteracts the generation of free radicals by inhibiting activity of the nitric oxide synthase.
Objective: This study is intended to assess the protective efficacy of melatonin against the γ radiation induced alterations in the morphological characteristics of sperm in the cauda epididymis of C57Bl/6 mice.
Materials and Methods: C57BL/6 male mice (n=12) were randomly divided into four groups: control, melatonin (100mg/kg body wt.), radiation (2Gy), melatonin (100mg/kg body wt.)+ radiation (2Gy). Control mice were sham irradiated, radiation grouped mice received 2 Gy of gamma irradiation, melatonin grouped mice received 100mg/kg body wt. of melatonin intra-peritonealy, Melatonin + radiation received 100 mg/kg body wt. of melatonin 30 min prior to 2Gy of gamma irradiation. All the mice were sacrificed 24 hr post irradiation and cauda epididymis was collected.
Results: In this study, various abnormal sperm shapes (amorphous heads, hook-less, mid-piece defect, pinheads, coiled tails, and tail-less) were observed after exposure to γ irradiation (2 Gy), treatment with melatonin alone, and after treatment with melatonin and γ-irradiation (100 mg/kg + 2 Gy). The higher rate of abnormal sperm forms was observed in the γ-irradiated mice compared with the melatonin + γ-irradiation treated mice (p<0.05). In addition, the number of sperm with amorphous heads and coiled tails was significantly increased after irradiation. Melatonin significantly (p<0.05) decreased the number of sperm with amorphous heads and coiled tails. This study suggests that melatonin can effectively reduce the effects of 2Gy radiation in sperms of C57BL/6 mice.
25 minutes 12 seconds